Distinguished Scientist Seminar Series featuring Alison Tebo, PhD – “Development of fluorescent tags and biosensors for imaging cellular biochemistry”

HYBRID

Distinguished Scientist Seminar Series

Alison Tebo, PhD
Group Leader
Howard Hughes Medical Institute – Janelia Research Campus

Location: New Research Building Auditorium and via Zoom: https://georgetown.zoom.us/j/94358558548

Title & Abstract: 

“Development of fluorescent tags and biosensors for imaging cellular biochemistry”

Fluorescence imaging plays a vital role in cell and molecular biology, with fluorescent proteins like GFP revolutionizing microscopy by offering precise control over tagged constructs. Fluorescent biosensors extend this capability, providing real-time insights into cellular biochemistry. Fluorescent biosensors combine molecular recognition with fluorescence to spatiotemporally resolve biochemical events of interest. However, their development often involves time-consuming and serendipitous processes of mutagenesis and screening. To enhance efficiency, we integrate machine learning and high-throughput assays for a more systematic exploration of the functional landscape of fluorescent biosensors. Additionally, we investigate alternative hybrid systems, such as the Fluorescence-activation and Absorption Shifting Tag (FAST), which uses a small protein tag to dynamically activate the fluorescence of a small molecule chromophore.
These hybrid chemical-genetic systems, including FAST, present unique opportunities for engineering, leveraging both protein and fluorogen independently. Notably, they also offer advantages, such as independence from oxygen requirements. In my seminar, I will discuss our techniques for imaging multiple targets and monitoring protein-protein interactions, showcasing the potential applications of these approaches in fluorescence imaging across diverse biological contexts.